Explore how your gene looks and see where peptides come from when you digest proteins with different enzymes. You can see your gene's structure with all different transcript variants, find exactly which peptides you'll get from each transcript, choose your enzyme to compare how different proteases cut your protein, and interactively explore the data by hovering, zooming, and clicking to dig deeper.
Discover how alternative splicing creates different peptides. Find out which peptides are unique to specific splice variants! Browse all splicing events to see every way your gene gets spliced, investigate specific events by clicking to dive deep into individual splicing changes, compare splice variants with side-by-side peptide comparison between versions, and see the big picture by visualizing all splicing differences at once.
Browse the full list of peptides for the selected transcript pair.
A text summary highlighting the key differences in peptide content.
Detailed breakdown of all peptides and their AS event associations. Junction-spanning peptides show multiple coordinate ranges.
Analyze which transcript isoforms you can detect in your mass spec experiments by comparing peptides across all transcript variants! See all isoforms at once to compare peptides across every transcript variant, categorize peptides as universal, shared, and unique, visualize gene structure with exon and CDS boundaries with peptide mapping, and find isoform-specific signatures by discovering which peptides are unique to specific isoforms.
Select a specific isoform to highlight and analyze its unique, shared, and universal peptides. Focus on one isoform to highlight and analyze its peptides with color-coded specificity for unique, shared, and universal peptides, get detailed statistics with comprehensive specificity analysis, and download your analysis results.
Select 2 to 8 isoforms to visualize their structures side by side and analyze shared, unique, and universal peptides. Compare multiple isoforms by analyzing 2-8 transcript variants with side-by-side visualization to see structural differences, analyze shared peptides to identify universal, shared, and unique peptides, and get detailed overlap statistics with comparative summary.
Compare peptide coverage across multiple enzymes for a single isoform. Identify the best enzyme combination for detecting your target isoform! Focus on one isoform by selecting a specific transcript variant for detailed analysis, see coverage differences across up to 6 proteases with multi-enzyme comparison, compare peptide maps and coverage statistics with side-by-side visualization, and optimize your experiments by finding the best enzymes for your proteomics workflow.
Combine the power of multi-isoform and multi-enzyme analysis! Generate comprehensive peptide coverage matrices.
Select 2-8 isoforms AND 2-6 enzymes for comprehensive combinations, see all isoform×enzyme combinations with matrix visualization, use advanced filtering for universal, shared, and unique peptides, and get comprehensive analysis by dimension with coverage statistics.
Shows exon/CDS structure with peptide overlays
Analyze your novel transcript sequences in 3 simple steps:
Upload RNA-seq transcripts, long-read sequences, or predicted isoforms to discover novel proteins and compare them with known gene variants.
Got a specific peptide sequence? Use BLASTP to search through 20,000+ genes and find homologous matches with detailed statistics! This tool is BLASTP powered with industry-standard protein homology search, provides rich statistics including E-values, bit scores, and identity percentages, offers flexible matching to find exact matches and similar sequences, has customizable parameters to adjust sensitivity and specificity, and lets you jump right into full gene analysis by clicking results.
Click a result above, then navigate to detailed isoform analysis:
Analyze alternative splicing events from rMATS output files. Supports SE, A3SS, A5SS, MXE, and RI event types with protein translation and functional prediction.
Analyze alternative splicing events from SplAdder GFF3 output files. Supports SE, A3SS, A5SS, MXE, and RI event types with protein translation and functional prediction.